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KMID : 1005220090240010071
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Journal of Embryo Transfer
2009 Volume.24 No. 1 p.71 ~ p.76
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Production of Piglet Derived from In Vitro Produced Porcine Early Embryos
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Choi Chang-Yong
Kim Hyun-Jong
Cho Sang-Rae
Yeon Seong-Heum
Han Man-Hye
Kim Jae-Bum
Kim Sung-Jae
Kang Da-Won
Son Dong-Soo
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Abstract
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It is not easy for porcine embryos produced by in vitro systems to develop into blastocysts with high quality. To solve this problem, many researchers have developed novel culture methods. However, the formation of blastocysts with high quality is still low. In this study, we aimed to produce piglet following transfer of in vitro produced early embryos ( cell stage embryos) or morula and blastocyst. The cell stage embryos were transferred to five estrus-synchronized recipients (200 embryos per recipient). One of the five sows farrowed three piglets, which contain two live piglets and one dead piglet, 114 days after embryo transfer. However, two recipients transferred with morula and blastocysts did not farrow. Microsatellite analysis confirmed that the genomic DNA of two live piglets were not genetically identical to that of the recipient. These results indicate that it is possible to obtain piglets by transfer of early embryos produced by in vitro production (IVP) systems.
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KEYWORD
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porcine, piglet, embryo transfer, microsatellite, IVP
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